Ultrasonication can help identify biofilm-protected bacterial colonization on orthopaedic implants that show no overt signs of infection.
Glycocalyx-producing bacteria have been observed on orthopaedic devices that were removed for reasons other than infection. It has been suggested that the bacteria can adhere to the device surfaces within a biofilm and elude standard culture techniques.
Larry L. Nguyen, MD, at Ortho Arkansas, in Little Rock, Arkasas, United States, and colleagues adapted previously used ultrasonication protocols for disrupting surface biofilms, to look for bacterial colonisation on orthopaedic devices that were removed from patients who had no clinical evidence of infection.
The study involved patients undergoing revision total joint arthroplasty of the hip or knee. During surgery, the femoral component removed during surgery and a control femoral implant were placed in separate sterile bags of saline and then put in an ultrasonication bath for 30 minutes at 60 Hz.
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